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    Structured Review

    ATCC ev d68 strains us mo
    Ev D68 Strains Us Mo, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enterovirus+d68+ev+d68+strains+us+mo/Enterovirus+D68+%3BStrain%3A+US%2FMO%2F14-18947/pmc12593654-247-1-23
    Average 93 stars, based on 4 article reviews
    ev d68 strains us mo - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Standard Deviation:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Activity Assay:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Plaque Assay:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Incubation:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Concentration Assay:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Positive Control:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Recombinant:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Ligand Binding Assay:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Sequencing:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Mutagenesis:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Control:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Infection:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Virus:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Staining:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cellswere cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/1418953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EVA71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced fromATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.Cells were cultured at 37 °C in a humidified incubator with 5% CO2 and routinely screened to confirm the absence of mycoplasma contamination.. Enterovirus D68 (EV-D68) strains US/MO/14-18947, US/KY/14-18953, US/MO/14-18949, US/IL/14-18952, and US/IL/14-18956 (ATCC); Coxsackievirus B3 (CVB3) Nancy strain (VR-30); Enterovirus A71 (EV-A71) Tainan/4643/1998 (BEI Resources, NR-471); Coxsackievirus A6 (CVA6, VR-1801); and human rhinovirus 16 (RV16, VR-283) were sourced from ATCC or BEI Resources.. Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.Coxsackievirus A16 (CVA16) and poliovirus type 1 were provided by Dr. William Nix (CDC) under a material transfer agreement.



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    HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus D68, and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.

    Journal: bioRxiv

    Article Title: Paramyxovirus matrix proteins modulate host cell translation via exon-junction complex interactions in the cytoplasm

    doi: 10.1101/2024.09.05.611502

    Figure Lengend Snippet: HEK-293T cells were transfected with siRNA pools targeting eIF4A3, Y14, MAGOH, or non-targeting control siRNAs (NC), respectively. At 48 hrs post-transfection, cells were inoculated with the designated virus (A) HPIV3, (B) Cedar, (C) MuV, (D) NDV, (E) Influenza A (A/WSN/1933), (F) Enterovirus D68, and (G) SARA-CoV2 at a multiplicity of infection (m.o.i.) of 0.01. The titers of infectious supernatants were determined on Vero-CCL81 cells using a 10-fold serial dilution at the indicated time points. For each virus, the expression levels of endogenous eIF4AIII, Y14, and MAGOH, along with infection control for viral protein or EGFP reporter, were analyzed by immunoblotting; results shown beside each panel confirm the knockdown of target proteins and validate virus infection. Symbols represent the data points from biological triplicates. Bars represent the mean of the triplicates. Statistical significance was determined by two-way ANOVA with Dunnett multiple comparison test. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001; ns, not significant. Immunoblottings are shown beside each to determine the knockdown of target proteins and controls for virus infection.

    Article Snippet: EV D68 strain US/MO/14-18947 (ATCC, VR-1823) were amplified in RD cells.

    Techniques: Transfection, Control, Virus, Infection, Serial Dilution, Expressing, Western Blot, Knockdown, Comparison